A cytological efficiency evaluation study of rare earth and carbon based material against breast cancer cells

Abstract

Cancer is a denying disease and among a number of cancers types, breast cancer is usual in females, and affect the worldwide. For these reason, the work presented here to show the cytological and apoptotic evaluation of breast (MCF-7) cancer cells with functional carbon nanotubes (FCNTs) and rare earth material (REE) neodymium oxide. At initial, the CNTs were functionalized with acid treatment method, whereas neodymium oxide nanorods (Nd2O3NRs) were synthesized and characterized. The Fourier transform infrared (FTIR) was used to check the functional characteristics of CNTs, whereas crystalline property, particle size and phase of Nd2O3NRs were confirmed via X-ray diffraction pattern (XRD) respectively. The morphologies of nanostructures were observed via scanning electron microscopy (SEM) and transmission electron microscopy (TEM) correspondingly. The cytological efficiencies of cancer cells were evaluated with FCNTs and Nd2O3NRs at varied concentrations (1, 2, 5, 10, 25, 50 and 100 μg/mL) verified via MTT and NRU assays. The ROS was enhanced in Nd2O3NRs as compared to CNTs with control. The gene expression study were also conducted and it unveils that the level of mRNA with Nd2O3NRs were upregulated and it depicts the apoptosis in cells. The study designates that the Nd2O3NRs are more efficient as compared to the functional CNTs with MCF-7 cancer cell.

Keywords

• Functional CNTs
• Neodymium oxide
• MCF-7 cells
• ROS
• RT-PCR