Programmable biomolecular switches for rewiring flux in Escherichia coli

Cong Gao; Jianshen Hou; Peng Xu; Liang Guo; Xiulai Chen; Guipeng Hu; Chao Ye; Harley Edwards; Jian Chen; Wei Chen; Liming Liu

doi:10.1038/s41467-019-11793-7

Nature Communications (Aug 2019)

Programmable biomolecular switches for rewiring flux in Escherichia coli

Cong Gao,
Jianshen Hou,
Peng Xu,
Liang Guo,
Xiulai Chen,
Guipeng Hu,
Chao Ye,
Harley Edwards,
Jian Chen,
Wei Chen,
Liming Liu

Affiliations

Cong Gao: State Key Laboratory of Food Science and Technology, Jiangnan University
Jianshen Hou: State Key Laboratory of Food Science and Technology, Jiangnan University
Peng Xu: Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County
Liang Guo: State Key Laboratory of Food Science and Technology, Jiangnan University
Xiulai Chen: State Key Laboratory of Food Science and Technology, Jiangnan University
Guipeng Hu: State Key Laboratory of Food Science and Technology, Jiangnan University
Chao Ye: State Key Laboratory of Food Science and Technology, Jiangnan University
Harley Edwards: Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County
Jian Chen: Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University
Wei Chen: State Key Laboratory of Food Science and Technology, Jiangnan University
Liming Liu: State Key Laboratory of Food Science and Technology, Jiangnan University

DOI: https://doi.org/10.1038/s41467-019-11793-7
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

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Current flux rewiring technologies in metabolic engineering are mainly transcriptional regulation. Here, the authors build two sets of controllable protein units using engineered viral proteases and proteolytic signals, and utilize for increasing titers of shikimate and D-xylonate in E. coli.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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