A Novelly-Spatiotemporal Characterization of the Disease Course in the MNU-Induced Retinitis Pigmentosa Model

Abstract

Weiming Yan,1,* Qiurui He,2,* Pan Long,3,* Lei Zhang,4 Haiyan Wang,4 Tao Chen5 1Department of Ophthalmology, Fuzong Clinical Medical College of Fujian Medical University, Dongfang Hospital Affiliated to Xiamen University, Fuzhou, People’s Republic of China; 2Department of Cardiovascular Intervention, The Third Hospital of Zhangzhou, Zhangzhou, People’s Republic of China; 3Department of Ophthalmology, The General Hospital of Western Theatre Command, PLA, Chengdu, People’s Republic of China; 4Department of Ophthalmology, The Shaanxi Eye Hospital, Xi’an People’s Hospital (Xi’an Fourth Hospital), Xi’an, People’s Republic of China; 5Center of Clinical Aerospace Medicine, Air Force Military Medical University, Xi’an, People’s Republic of China*These authors contributed equally to this workCorrespondence: Weiming Yan; Tao Chen, Email [email protected]; [email protected]: With the aids of ophthalmic imaging techniques for animals, the spatiotemporal characterization of MNU-induced retinitis pigmentosa (RP) rats were performed.Methods: Sprague-Dawley (SD) rats were randomly divided into normal group (N), MNU-low-dose group (L) and MNU-high-dose group (H). Rats in the L and H group were given intraperitoneally injection with 40 and 60 mg/kg of MNU, a kind of alkylating agent, respectively. The body weight, electroretinogram (ERG) and retinal structure were observed on day one (D1), D3, and D7 after MNU administration. FFA, OCT, TUNEL staining, and immunostaining of Iba1 were also performed.Results: After MNU injection, the weight and ERG amplitudes of rats in both L and H groups decreased gradually, compared to those of the normal group (P

Keywords

• retinitis pigmentosa
• animal model
• n-methyl-n-nitrosourea
• microglia.