Phage‐displayed heptapeptide sequence conjugation significantly improves the specific targeting ability of antimicrobial peptides against Staphylococcus aureus
Tao Wang,
Peng Tan,
Qi Tang,
Chenlong Zhou,
Yakun Ding,
Shenrui Xu,
Mengda Song,
Huiyang Fu,
Yucheng Zhang,
Xiaohui Zhang,
Yueyu Bai,
Zhihong Sun,
Xi Ma
Affiliations
Tao Wang
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology China Agricultural University Beijing China
Peng Tan
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology China Agricultural University Beijing China
Qi Tang
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology China Agricultural University Beijing China
Chenlong Zhou
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology China Agricultural University Beijing China
Yakun Ding
Key Laboratory of Innovative Utilization of Indigenous Cattle and Sheep Germplasm Resources (Co‐construction by Ministry and Province), Ministry of Agriculture and Rural Affairs Zhengzhou University Zhengzhou China
Shenrui Xu
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology China Agricultural University Beijing China
Mengda Song
Key Laboratory of Innovative Utilization of Indigenous Cattle and Sheep Germplasm Resources (Co‐construction by Ministry and Province), Ministry of Agriculture and Rural Affairs Zhengzhou University Zhengzhou China
Huiyang Fu
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology China Agricultural University Beijing China
Yucheng Zhang
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology China Agricultural University Beijing China
Xiaohui Zhang
Luoyang Key Laboratory of Animal Genetic and Breeding, College of Animal Science Henan University of Science and Technology Luoyang China
Yueyu Bai
Key Laboratory of Innovative Utilization of Indigenous Cattle and Sheep Germplasm Resources (Co‐construction by Ministry and Province), Ministry of Agriculture and Rural Affairs Zhengzhou University Zhengzhou China
Zhihong Sun
Laboratory for Bio‐Feed and Molecular Nutrition, Department of Animal Science and Technology Southwest University Chongqing China
Xi Ma
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology China Agricultural University Beijing China
Abstract Broad‐spectrum antibacterial drugs often lack specificity, leading to indiscriminate bactericidal activity, which can disrupt the normal microbial balance of the host flora and cause unnecessary cytotoxicity during systemic administration. In this study, we constructed a specifically targeted antimicrobial peptide against Staphylococcus aureus by introducing a phage‐displayed peptide onto a broad‐spectrum antimicrobial peptide and explored its structure–function relationship through one‐factor modification. SFK2 obtained by screening based on the selectivity index and the targeting index showed specific killing ability against S. aureus. Moreover, SFK2 showed excellent biocompatibility in mice and piglet, and demonstrated significant therapeutic efficacy against S. aureus infection. In conclusion, our screening of phage‐derived heptapeptides effectively enhances the specific bactericidal ability of the antimicrobial peptides against S. aureus, providing a theoretical basis for developing targeted antimicrobial peptides.
