Gold nanoparticle-based immunochromatographic assay for detection Pseudomonas aeruginosa in water and food samples

Lu Zeng; Lingling Guo; Zhongxing Wang; Xinxin Xu; Hongliu Ding; Shanshan Song; Liguang Xu; Hua Kuang; Chuanlai Xu

Food Chemistry: X (Mar 2021)

Gold nanoparticle-based immunochromatographic assay for detection Pseudomonas aeruginosa in water and food samples

Lu Zeng,
Lingling Guo,
Zhongxing Wang,
Xinxin Xu,
Hongliu Ding,
Shanshan Song,
Liguang Xu,
Hua Kuang,
Chuanlai Xu

Affiliations

Lu Zeng: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China
Lingling Guo: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China
Zhongxing Wang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China
Xinxin Xu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China
Hongliu Ding: Suzhou Product Quality Supervision Inspection, 1368 Wuzhong Avenue, Suzhou 215104, China
Shanshan Song: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China
Liguang Xu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; Corresponding authors at: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China.
Hua Kuang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China
Chuanlai Xu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China; Corresponding authors at: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China.

Journal volume & issue: Vol. 9
p. 100117

Abstract

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Pseudomonas aeruginosa (P. aeruginosa) is the common infection-causing bacterial pathogen. Conventional methods for the detection of P. aeruginosa are time-consuming, and therefore, a more rapid analytical method is required. Here, monoclonal antibodies (Mabs) against P. aeruginosa (CICC 10419) were prepared and based on paired Mabs, an immunochromatographic assay (ICA) was developed. The ICA strip showed a limit of detection of 2.41 × 104 CFU/mL and the linear range of detection was 3.13 × 104-1.0 × 106 CFU/mL. No cross-reactivity was observed when other common Gram-negative and Gram-positive bacteria were used. The analytical performance of the ICA strip indicated that the developed ICA had good specificity and stability. Moreover, the feasibility of the ICA strip was verified by detecting P. aeruginosa (CICC 10419) in spiked water and food samples. The ICA strip could detect samples contaminated with a low-level of P. aeruginosa (CICC 10419) after 8 h enrichment.

Published in Food Chemistry: X

ISSN: 2590-1575 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply; Technology: Chemical technology: Food processing and manufacture
Website: https://www.journals.elsevier.com/food-chemistry-x

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