A negative magnetoresistance (MR) was observed at room temperature in a Co/Py transverse “coercivity heterostructures” configuration thin film, and it can be explained by domain wall scattering at the boundaries of the Co and Py. We point out that the novel “soft” and “hard” transverse “coercivity heterostructures” not only can be used to study domain wall resistance of variety magnetic materials, but also have potential application in fabricating domain wall devices.