Similarity and characterization of structural and functional neural connections within species under isoflurane anesthesia in the common marmoset
Daisuke Yoshimaru,
Tomokazu Tsurugizawa,
Junichi Hata,
Kanako Muta,
Takuto Marusaki,
Naoya Hayashi,
Shuhei Shibukawa,
Kei Hagiya,
Hideyuki Okano,
Hirotaka James Okano
Affiliations
Daisuke Yoshimaru
Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan; National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan; Faculty of Engineering, University of Tsukuba, Tsukuba, Ibaraki, Japan; Department of Radiology, Tokyo Medical University, Tokyo, Japan; Department of Physiology, Keio University School of Medicine, Tokyo, Japan
Tomokazu Tsurugizawa
Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan; National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan; Faculty of Engineering, University of Tsukuba, Tsukuba, Ibaraki, Japan
Junichi Hata
Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan; Graduate School of Human Health Sciences, Tokyo Metropolitan University, Tokyo, Japan; Department of Physiology, Keio University School of Medicine, Tokyo, Japan
Kanako Muta
Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan; Graduate School of Human Health Sciences, Tokyo Metropolitan University, Tokyo, Japan
Takuto Marusaki
Graduate School of Human Health Sciences, Tokyo Metropolitan University, Tokyo, Japan
Naoya Hayashi
Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan; Graduate School of Human Health Sciences, Tokyo Metropolitan University, Tokyo, Japan; Department of Radiology, Tokyo Medical University, Tokyo, Japan
Shuhei Shibukawa
Department of Radiology, Tokyo Medical University, Tokyo, Japan; Faculty of Health Science, Department of Radiological Technology, Juntendo University, Tokyo, Japan
Kei Hagiya
Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan
Hideyuki Okano
Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan; Department of Physiology, Keio University School of Medicine, Tokyo, Japan; Corresponding authors at: Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Hirotaka James Okano
Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan; Corresponding authors at: Division of Regenerative Medicine, The Jikei University School of Medicine, 3-25-8, Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
The common marmoset is an essential model for understanding social cognition and neurodegenerative diseases. This study explored the structural and functional brain connectivity in a marmoset under isoflurane anesthesia, aiming to statistically overcome the effects of high inter-individual variability and noise-related confounds such as physiological noise, ensuring robust and reliable data. Similarities and differences in individual subject data, including assessments of functional and structural brain connectivities derived from resting-state functional MRI and diffusion tensor imaging were meticulously captured. The findings highlighted the high consistency of structural neural connections within the species, indicating a stable neural architecture, while functional connectivity under anesthesia displayed considerable variability. Through independent component and dual regression analyses, several distinct brain connectivities were identified, elucidating their characteristics under anesthesia. Insights into the structural and functional features of the marmoset brain from this study affirm its value as a neuroscience research model, promising advancements in the field through fundamental and translational studies.