Frontiers in Microbiology (Dec 2019)
Baculovirus Utilizes Cholesterol Transporter NIEMANN–Pick C1 for Host Cell Entry
- Zhihong Li,
- Zhihong Li,
- Youpeng Fan,
- Youpeng Fan,
- Junhong Wei,
- Junhong Wei,
- Junhong Wei,
- Xionge Mei,
- Qiang He,
- Qiang He,
- Yonghua Zhang,
- Tian Li,
- Tian Li,
- Tian Li,
- Mengxian Long,
- Mengxian Long,
- Mengxian Long,
- Jie Chen,
- Jie Chen,
- Jie Chen,
- Jialing Bao,
- Jialing Bao,
- Jialing Bao,
- Guoqing Pan,
- Guoqing Pan,
- Guoqing Pan,
- Chunfeng Li,
- Chunfeng Li,
- Chunfeng Li,
- Zeyang Zhou,
- Zeyang Zhou,
- Zeyang Zhou,
- Zeyang Zhou
Affiliations
- Zhihong Li
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Zhihong Li
- Department of Microbiology, Guizhou University of Traditional Chinese Medicine, Guiyang, China
- Youpeng Fan
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Youpeng Fan
- Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China
- Junhong Wei
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Junhong Wei
- Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China
- Junhong Wei
- Key Laboratory for Sericulture Functional Genomics Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China
- Xionge Mei
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Qiang He
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Qiang He
- Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China
- Yonghua Zhang
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Tian Li
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Tian Li
- Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China
- Tian Li
- Key Laboratory for Sericulture Functional Genomics Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China
- Mengxian Long
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Mengxian Long
- Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China
- Mengxian Long
- Key Laboratory for Sericulture Functional Genomics Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China
- Jie Chen
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Jie Chen
- Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China
- Jie Chen
- Key Laboratory for Sericulture Functional Genomics Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China
- Jialing Bao
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Jialing Bao
- Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China
- Jialing Bao
- Key Laboratory for Sericulture Functional Genomics Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China
- Guoqing Pan
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Guoqing Pan
- Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China
- Guoqing Pan
- Key Laboratory for Sericulture Functional Genomics Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China
- Chunfeng Li
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Chunfeng Li
- Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China
- Chunfeng Li
- Key Laboratory for Sericulture Functional Genomics Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China
- Zeyang Zhou
- State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China
- Zeyang Zhou
- Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China
- Zeyang Zhou
- Key Laboratory for Sericulture Functional Genomics Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China
- Zeyang Zhou
- College of Life Sciences, Chongqing Normal University, Chongqing, China
- DOI
- https://doi.org/10.3389/fmicb.2019.02825
- Journal volume & issue
-
Vol. 10
Abstract
The dual roles of baculovirus for the control of natural insect populations as an insecticide, and as a tool for foreign gene expression and delivery, have called for a comprehensive understanding of the molecular mechanisms governing viral infection. Here, we demonstrate that the Bombyx mori Niemann-Pick C1 (BmNPC1) is essential for baculovirus infection in insect cells. Both pretreatment of B. mori embryonic cells (BmE) with NPC1 antagonists (imipramine or U18666A) and down-regulation of NPC1 expression resulted in a significant reduction in baculovirus BmNPV (B. mori nuclear polyhedrosis virus) infectivity. Disruption of BmNPC1 could decrease viral entry (2 hpi) rather than reduce the viral binding to the BmE cells. Furthermore, our results showed that NPC1 domain C binds directly and specifically to the viral glycoprotein GP64, which is responsible for both receptor binding and fusion. Antibody blocking assay also revealed that the domain C specific polyclonal antibody inhibited BmNPV infection, indicating that NPC1 domain C most likely plays a role during viral fusion in endosomal compartments. Our results, combined with previous studies identifying an essential role of human NPC1 (hNPC1) in filovirus infection, suggest that the glycoprotein of several enveloped viruses possess a shared strategy of exploiting host NPC1 proteins during virus intracellular entry events.
Keywords
