Antibiofilm Activity of Acidic Phospholipase Isoform Isolated from <i>Bothrops erythromelas</i> Snake Venom
Ellynes Nunes,
Breno Frihling,
Elizângela Barros,
Caio de Oliveira,
Newton Verbisck,
Taylla Flores,
Augusto de Freitas Júnior,
Octávio Franco,
Maria de Macedo,
Ludovico Migliolo,
Karla Luna
Affiliations
Ellynes Nunes
Postgraduate Program in Cellular and Molecular Biology—Federal University of Paraíba, João Pessoa, PB 58051-900, Brazil
Breno Frihling
S-Inova Biotech, Postgraduate Program in Biotechnology—Dom Bosco Catholic University, Campo Grande, MS 79117-010, Brazil
Elizângela Barros
S-Inova Biotech, Postgraduate Program in Biotechnology—Dom Bosco Catholic University, Campo Grande, MS 79117-010, Brazil
Caio de Oliveira
Protein Purification Laboratory and its Biological Functions, Faculty of Medicine, FAMED, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS 79603-011, Brazil
Newton Verbisck
Embrapa Beef Cattle, Campo Grande, MS 79106-550, Brazil
Taylla Flores
Postgraduate Program in Cellular and Molecular Biology—Federal University of Paraíba, João Pessoa, PB 58051-900, Brazil
Augusto de Freitas Júnior
Postgraduate Program in Cellular and Molecular Biology—Federal University of Paraíba, João Pessoa, PB 58051-900, Brazil
Octávio Franco
S-Inova Biotech, Postgraduate Program in Biotechnology—Dom Bosco Catholic University, Campo Grande, MS 79117-010, Brazil
Maria de Macedo
Protein Purification Laboratory and its Biological Functions, Faculty of Medicine, FAMED, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS 79603-011, Brazil
Ludovico Migliolo
Postgraduate Program in Cellular and Molecular Biology—Federal University of Paraíba, João Pessoa, PB 58051-900, Brazil
Karla Luna
Postgraduate Program in Cellular and Molecular Biology—Federal University of Paraíba, João Pessoa, PB 58051-900, Brazil
Introduction: Bacterial resistance is a worldwide public health problem, requiring new therapeutic options. An alternative approach to this problem is the use of animal toxins isolated from snake venom, such as phospholipases A2 (PLA2), which have important antimicrobial activities. Bothropserythromelas is one of the snake species in the northeast of Brazil that attracts great medical-scientific interest. Here, we aimed to purify and characterize a PLA2 from B. erythromelas, searching for heterologous activities against bacterial biofilms. Methods: Venom extraction and quantification were followed by reverse-phase high-performance liquid chromatography (RP-HPLC) in C18 column, matrix-assisted ionization time-of-flight (MALDI-ToF) mass spectrometry, and sequencing by Edman degradation. All experiments were monitored by specific activity using a 4-nitro-3-(octanoyloxy) benzoic acid (4N3OBA) substrate. In addition, hemolytic tests and antibacterial tests including action against Escherichiacoli, Staphylococcusaureus, and Acinetobacterbaumannii were carried out. Moreover, tests of antibiofilm action against A. baumannii were also performed. Results: PLA2, after one purification step, presented 31 N-terminal amino acid residues and a molecular weight of 13.6564 Da, with enzymatic activity confirmed in 0.06 µM concentration. Antibacterial activity against S. aureus (IC50 = 30.2 µM) and antibiofilm activity against A. baumannii (IC50 = 1.1 µM) were observed. Conclusions: This is the first time that PLA2 purified from B. erythromelas venom has appeared as an alternative candidate in studies of new antibacterial medicines.
