PENGARUH KONSENTRASI EKSTRAK ETANOL BUAH PARE (Momordica charantia L) TERHADAP PERTUMBUHAN BAKTERI Shigella dysenteriae SECARA IN VITRO

Abstract

One of the gastrointestinal diseases are dysentery, which is characterized by loose, watery stools with mucus and blood of more than 3 times per day. Dysentery caused by Shigella dysenteriae bacteria, the disease can be treated with an antibacterial nutritious plants, one of which is bitter melon. Plant part used is fruit. Bitter melon fruit (Momordica charantia L) contains flavonoids which efficacious as antibacterials. This study aims to determine the effect of extract concentrations against the bacteria Shigella dysenteriae. Antibacterial activity was tested using the disc diffusion method with extract concentrations of 10%, 25% and 50%. Clear zone formed around the paper discs showed inhibition zone. Results showed the ethanol extract of bitter melon extract at concentration 10%, 25% and 50% showed their antibacterial activity against bacteria Shigella dysenteriae with average diameter of inhibition zone 8.3 mm, 9.3 mm and 11.7 mm. Increased concentration of bitter melon fruit extract could increased inhibition zone.

Keywords

• Concentration
• ethanol extract
• bitter melon
• bacteria Shigella dysenteriae