Frontiers in Microbiology (Nov 2022)

The protective immunity induced by intranasally inoculated serotype 63 chimpanzee adenovirus vector expressing human respiratory syncytial virus prefusion fusion glycoprotein in BALB/c mice

  • Lei Huang,
  • Mei-Qing Liu,
  • Chang-Qing Wan,
  • Ning-Ning Cheng,
  • Yan-Bin Su,
  • Yan-Peng Zheng,
  • Xiang-Lei Peng,
  • Jie-Mei Yu,
  • Yuan-Hui Fu,
  • Jin-Sheng He

DOI
https://doi.org/10.3389/fmicb.2022.1041338
Journal volume & issue
Vol. 13

Abstract

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Human respiratory syncytial virus (RSV) is a ubiquitous pediatric pathogen causing serious lower respiratory tract disease worldwide. No licensed vaccine is currently available. In this work, the coding gene for mDS-Dav1, the full-length and prefusion conformation RSV fusion glycoprotein (F), was designed by introducing the stabilized prefusion F (preF) mutations from DS-Cav1 into the encoding gene of wild-type RSV (wtRSV) F protein. The recombinant adenovirus encoding mDS-Cav1, rChAd63-mDS-Cav1, was constructed based on serotype 63 chimpanzee adenovirus vector and characterized in vitro. After immunizing mice via intranasal route, the rChAd63-mDS-Cav1 induced enhanced neutralizing antibody and F-specific CD8+ T cell responses as well as good immune protection against RSV challenge with the absence of enhanced RSV disease (ERD) in BALB/c mice. The results indicate that rChAd63-mDS-Cav1 is a promising mucosal vaccine candidate against RSV infection and warrants further development.

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