The Effect of Storage on the Protein Electrophoretic Pattern in Bovine Serum

Abstract

This study was aimed at the evaluation of the influence of storage under various conditions on the relative concentrations of major protein fractions and their proportion in bovine serum. Blood samples were taken from six dairy cattle of a low-land black spotted breed and its crossbreeds. The separated blood serum was fractioned into aliquots. One aliquot was analysed immediately after the separation without storage. The second aliquot was stored at 4 °C for 1 day, the remaining aliquots were kept frozen at -18 °C for 2, 7, and 21 days, and then analysed. Blood serum was analysed by agarose gel electrophoresis for the major protein fractions – albumin (%), alpha-globulins (%), beta-globulins (%), and gammaglobulins (%). Over time, the relative concentrations of albumin in bovine serum showed a tendency of significant decrease during the storage at -18 °C (p

Keywords

• storage
• electrophoresis
• protein fraction
• dairy cattle