Frontiers in Immunology (Jan 2022)
P311 Facilitates the Angiogenesis and Wound Healing Function of MSCs by Increasing VEGF Production
- Zhihui Liu,
- Zhihui Liu,
- Jiacai Yang,
- Jiacai Yang,
- Yunxia Chen,
- Yunxia Chen,
- Cheng Chen,
- Cheng Chen,
- Jue Wang,
- Jue Wang,
- Yew Mun Lee,
- Yew Mun Lee,
- Wenxia Zheng,
- Wenxia Zheng,
- Ruoyu Shang,
- Ruoyu Shang,
- Yuanyang Tang,
- Yuanyang Tang,
- Xiaorong Zhang,
- Xiaorong Zhang,
- Xiaohong Hu,
- Xiaohong Hu,
- Yong Huang,
- Yong Huang,
- Shiya Peng,
- Yih-Cherng Liou,
- Yih-Cherng Liou,
- Weifeng He,
- Weifeng He,
- Gaoxing Luo,
- Gaoxing Luo
Affiliations
- Zhihui Liu
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Zhihui Liu
- Department of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, China
- Jiacai Yang
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Jiacai Yang
- Department of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, China
- Yunxia Chen
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Yunxia Chen
- Department of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, China
- Cheng Chen
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Cheng Chen
- Department of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, China
- Jue Wang
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Jue Wang
- Department of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, China
- Yew Mun Lee
- Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore
- Yew Mun Lee
- National University of Singapore (NUS) Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore
- Wenxia Zheng
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Wenxia Zheng
- Department of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, China
- Ruoyu Shang
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Ruoyu Shang
- Department of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, China
- Yuanyang Tang
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Yuanyang Tang
- Academy of Biological Engineering, Chongqing University, Chongqing, China
- Xiaorong Zhang
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Xiaorong Zhang
- Department of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, China
- Xiaohong Hu
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Xiaohong Hu
- Department of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, China
- Yong Huang
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Yong Huang
- Department of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, China
- Shiya Peng
- Department of Dermatology, Xinqiao Hospital, Army Military Medical University, Chongqing, China
- Yih-Cherng Liou
- Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore
- Yih-Cherng Liou
- National University of Singapore (NUS) Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore
- Weifeng He
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Weifeng He
- Department of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, China
- Gaoxing Luo
- State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
- Gaoxing Luo
- Department of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, China
- DOI
- https://doi.org/10.3389/fimmu.2022.821932
- Journal volume & issue
-
Vol. 13
Abstract
As a potential clinical therapeutic cell for injured tissue repair, mesenchymal stem cells (MSCs) have attracted increasing attention. Enhancing the pro-healing function of MSCs has gradually become an essential topic in improving the clinical efficacy of MSCs. Recently, studies have shown that neuronal protein 3.1 (P311) plays a crucial role in promoting skin wound healing, suggesting P311 gene modification may improve the pro-healing function of MSCs. In this study, we demonstrated that increasing the in vivo expression of P311 could significantly enhance the ability of MSCs to lessen the number of inflammatory cells, increase the expression of IL10, reduce the levels of TNF-α and IFN-γ, increase collagen deposition, promote angiogenesis, and ultimately accelerate skin wound closure and improve the quality of wound healing. Importantly, we uncovered that P311 enhanced the pro-angiogenesis function of MSCs by increasing the production of vascular endothelial growth factor (VEGF) in vitro and in vivo. Mechanistically, we revealed that the mTOR signalling pathway was closely related to the regulation of P311 on VEGF production in MSCs. Together, our data displayed that P311 gene modification in MSCs augments their capabilities to promote skin wound closure, which might bring the dawn for its clinical application in the future.
Keywords
