Toxics (Jun 2022)

Biochemical and Metabolomic Responses of Antarctic Bacterium <i>Planococcus</i> sp. O5 Induced by Copper Ion

  • Ziyi Cheng,
  • Cuijuan Shi,
  • Xiujun Gao,
  • Xiaofei Wang,
  • Guangfeng Kan

DOI
https://doi.org/10.3390/toxics10060302
Journal volume & issue
Vol. 10, no. 6
p. 302

Abstract

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Heavy metal pollution in the Antarctic has gone beyond our imagination. Copper toxicity is a selective pressure on Planococcus sp. O5. We observed relatively broad tolerance in the polar bacterium. The heavy metal resistance pattern is Pb2+ > Cu2+ > Cd2+ > Hg2+ > Zn2+. In the study, we combined biochemical and metabolomics approaches to investigate the Cu2+ adaptation mechanisms of the Antarctic bacterium. Biochemical analysis revealed that copper treatment elevated the activity of antioxidants and enzymes, maintaining the bacterial redox state balance and normal cell division and growth. Metabolomics analysis demonstrated that fatty acids, amino acids, and carbohydrates played dominant roles in copper stress adaptation. The findings suggested that the adaptive mechanisms of strain O5 to copper stress included protein synthesis and repair, accumulation of organic permeable substances, up-regulation of energy metabolism, and the formation of fatty acids.

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