Transcriptomic Analysis Reveals the Dependency of Pseudomonas aeruginosa Genes for Double-Stranded RNA Bacteriophage phiYY Infection Cycle
Qiu Zhong,
Lan Yang,
Linlin Li,
Wei Shen,
Yang Li,
Huan Xu,
Zhuojun Zhong,
Ming Chen,
Shuai Le
Affiliations
Qiu Zhong
Department of Clinical Laboratory Medicine, Southwest Hospital, Army Medical University, Chongqing 400038, China; Department of Clinical Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing 400038, China
Lan Yang
Shanghai Institute of Phage, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
Linlin Li
Shanghai Institute of Phage, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
Wei Shen
Department of Microbiology, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China
Yang Li
Medical Center of Trauma and War Injury, Daping Hospital, Army Medical University, Chongqing 400038, China; State Key Laboratory of Trauma, Burns and Combined Injuries, Army Medical University, Chongqing 400038, China
Huan Xu
Department of Clinical Laboratory Medicine, Southwest Hospital, Army Medical University, Chongqing 400038, China
Zhuojun Zhong
Department of Microbiology, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China
Ming Chen
Department of Clinical Laboratory Medicine, Southwest Hospital, Army Medical University, Chongqing 400038, China; State Key Laboratory of Trauma, Burns and Combined Injuries, Army Medical University, Chongqing 400038, China; Corresponding author
Shuai Le
Department of Microbiology, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China; Corresponding author
Summary: Bacteriophage phiYY is currently the only double-stranded RNA (dsRNA) phage that infects Pseudomonas aeruginosa and is a potential candidate for phage therapy. Here we applied RNA-seq to investigate the lytic cycle of phiYY infecting P. aeruginosa strain PAO1r. About 12.45% (651/5,229) of the host genes were determined to be differentially expressed genes. Moreover, oxidative stress response genes katB and ahpB are upregulated 64- to 128-fold after phage infection, and the single deletion of each gene blocked phiYY infection, indicating that phiYY is extremely sensitive to oxidative stress. On the contrary, another upregulated gene PA0800 might constrain phage infection, because the deletion of PA0800 resulted in a 3.5-fold increase of the efficiency of plating. Our study highlights a complicated dsRNA phage-phage global interaction and raises new questions toward the host defense mechanisms against dsRNA phage and dsRNA phage-encoded hijacking mechanisms.
