OncoTargets and Therapy (Jan 2020)
lncRNA FLVCR-AS1 promotes osteosarcoma growth by targeting miR381-3p/CCND1
Abstract
Guang Yang, 1, 2 Fei He, 2 Hao Duan, 2 Jianlin Shen, 3 Qirong Dong 1 1Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; 2Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650200, China; 3Department of Orthopedics, Affiliated Hospital of Putian University, Putian 351100, ChinaCorrespondence: Dr. Qirong DongDepartment of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou 215004, ChinaTel +86-0512-68282030Email [email protected]: This article reports on FLVCR-AS1 effects on osteosarcoma (OS) growth.Methods: Tumor tissue and adjacent normal tissue of 48 OS patients were collected. HOS and 143B cells were transfected. Gene expression was examined with qRT-PCR and Western blot. CCK8 assays and cell cloning was performed to measure cell proliferation. Cell cycle and apoptosis were assessed. Luciferase-reporter gene assays and RNA pull-down tests were used to detect targeting relationships between genes.Results: Prominently higher FLVCR-AS1 expression was found in OS tissue and cells, and was associated with poor prognosis (P< 0.05, P< 0.01, or P< 0.001). Compared with the siCtrl group, 143B and HOS cells of the siFLVCR-AS1 group had significantly lower OD 450 values and clone numbers and obviously higher percentages of cells in the G 1 phase and apoptosis (P< 0.01 or P< 0.001). miR381-3p expression was directly inhibited by FLVCR-AS1, and CCND1 expression was directly suppressed by miR381-3p. Compared with the FLVCR-AS1 group, 143B cells of the FLVCR-AS1+ miR381-3p mimic group and FLVCR-AS1+ siCCND1 group showed remarkably lower OD 450 values and clone numbers obviously higher apoptosis and percentage of cells in the G 1 phase (P< 0.05, P< 0.01, or P< 0.001).Conclusion: FLVCR-AS1 promoted OS growth by upregulating CCND1 expression via downregulation of miR381-3p.Keywords: OS, FLVCR-AS1, miR381-3p, CCND1, proliferation, apoptosis
