Experimental study on the mechanism of curcumin inhibiting the proliferation of human hepatocellular carcinoma cells HepG2

Abstract

Objective: The purpose of this experiment is to study the effects of Curcumin (Cur) on the proliferation, apoptosis and cell cycle of human hepatocellular carcinoma HepG2 cells, and to explore its mechanism of inhibiting the proliferation of HepG2 cells. Methods: The experiment was divided into control group and Cur group. The HepG2 cultured human hepatoma cells were treated with Cur solution of different concentrations. The inhibitory effect of Cur on the proliferation of HepG2 cells was detected by MTT assay. The apoptosis rate and cell cycle distribution of CUR-induced HepG2 cells were detected by flow cytometry. Results: Compared with the control group, the growth of HepG2 cells was inhibited by Cur in a concentration-dependent manner. Apoptosis by flow cytometry. Results: Compared with the control group, the growth of HepG2 cells was inhibited by Cur in a concentration-dependent manner. Flow cytometry showed that the apoptosis rates of the control group and the Cur group were 0.3%, 18.0%, 24.7% and 86.9%, respectively, showing a dose-dependent relationship. Cell cycle detection showed that in the control group and Cur concentration groups, the proportion of S phase was 24.11%, 32.68%, 37.75%, 42.10%, respectively. The apoptosis percentage was most obvious in S phase, and cells were blocked in S phase. Compared with control group, with the increase of Cur concentration. Conclusion: Curcumin may inhibit the proliferation of human hepatoma cells HepG2 by preventing cell division and inducing cell apoptosis.