A non-integrated iPSC line (SDQLCHi042-A) from a boy suffering from familial combined hyperlipidemia with compound heterozygous mutations of lipoprotein lipase gene

Zilong Li; Xue Zhang; Xiaomei Li; Yanan Yang; Hongmei Xin; Xiaomeng Yang; Ning Liu; Zhongtao Gai; Yi Liu

Stem Cell Research (May 2021)

A non-integrated iPSC line (SDQLCHi042-A) from a boy suffering from familial combined hyperlipidemia with compound heterozygous mutations of lipoprotein lipase gene

Zilong Li,
Xue Zhang,
Xiaomei Li,
Yanan Yang,
Hongmei Xin,
Xiaomeng Yang,
Ning Liu,
Zhongtao Gai,
Yi Liu

Affiliations

Zilong Li: Jinan Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Xue Zhang: Jinan Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China; Neonatal Intensive Care Unit, Children's Medical Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, China
Xiaomei Li: Neonatal Intensive Care Unit, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Yanan Yang: Jinan Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Hongmei Xin: Jinan Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Xiaomeng Yang: Jinan Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Ning Liu: Jinan Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Zhongtao Gai: Jinan Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China; Corresponding author.
Yi Liu: Jinan Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China; Corresponding author.

Journal volume & issue: Vol. 53
p. 102313

Abstract

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In this study, peripheral blood monouclear cells (PBMCs) were donated from a boy suffering from familial combined hyperlipidemia confirmed by clinical and genetic diagnosis, which carried compound heterozygous mutations of lipoprotein lipase (LPL) gene. The induced pluripotent stem cell (iPSC) was generated with non-integrated episomal vectors carrying OCT4, SOX2, KLF4, BCL-XL and C-MYC. The iPSCs presented the morphology of pluripotent cells, highly expressed mRNA and protein of pluripotent markers, excellent differentiation potency in vitro and normal karyotype, and bore LPL gene mutations.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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