Journal of Inflammation Research (Jun 2023)
LncRNA THRIL Functions as a Marker for Carotid Artery Stenosis and Affects the Biological Function of Human Aortic Endothelial Cell
Abstract
Fei Wang,1 Ying Wang,2 Xichun Guo3 1Department of Neurosurgery, Affiliated Hospital of Weifang Medical University, Weifang, People’s Republic of China; 2Department of Medical Record Room, Affiliated Hospital of Weifang Medical University, Weifang, People’s Republic of China; 3Department of Pharmacy, Affiliated Hospital of Weifang Medical University, Weifang, People’s Republic of ChinaCorrespondence: Fei Wang, Department of Neurosurgery, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwen District, Weifang, Shandong, 261035, People’s Republic of China, Tel/Fax +86-536-3081100, Email [email protected]: Carotid artery restenosis (CAS) is a leading contributor to cerebrovascular diseases and one of the leading causes of death in the world. The purpose of this study was to assess the predictive efficiency of long non-coding RNA (lncRNA) TNFalpha-and hnRNP L-related immunoregulatory lncRNA (THRIL) and its association with the pathogenesis of CAS.Patients and Methods: The expression of THRIL was determined in patients with asymptomatic CAS and human aortic endothelial cell (HAEC) models induced by oxidized low-density lipoprotein (ox-LDL). The receiver operating characteristic (ROC) curve and Kaplan–Meier (K-M) drawings were constructed to predict the risk of poor prognosis in patients with CAS. The cell proliferation, death rate, and inflammation were detected by 3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), flow cytometry, and enzyme-linked immunosorbent assay (ELISA) assays.Results: The relative expression of THRIL was elevated in patients with asymptomatic CAS. The findings of ROC curve indicated that THRIL had a predictive possibility on CAS. K-M finding and Cox regression analysis showed that the expression of THRIL and the degree of CAS were independent risk factors for poor prognosis in patients with CAS. THRIL was up-expressed in HAECs induced by ox-LDL. Down-regulation of THRIL could promote the proliferation of HAECs, inhibit cell apoptosis, and restrict cell inflammation.Conclusion: THRIL was a diagnostic and prognostic biomarker in CAS and played an important role in regulating the proliferation, apoptosis, and inflammation of HAECs induced by ox-LDL.Keywords: THRIL, carotid artery restenosis, prognosis, diagnosis, proliferation
