Impact of IL-21 Gene Polymorphisms (rs2055979) and the Levels of Serum IL-21 on the Risk of Multiple Sclerosis

Abstract

Multiple sclerosis (MS) is a chronic autoimmune disorder of the central nervous system. Genetic and environmental factors have important roles in the induction, onset, and progression of MS. In this study, the IL-21 genotype (rs2055979) (G/T) in Iraqi MS patients was compared with a healthy control group to investigate the possible association of any particular genotype or allele with multiple sclerosis. This study included 70 patients with relapsing-remitting MS and 50 healthy individuals as control. Following the extraction of genomic DNA, polymerase chain reaction-restriction fragment length polymorphism, the frequencies of genotypes, and alleles were calculated and statistically analyzed. The results of the study revealed a significant reduction in the distribution of the wild homozygous genotype (GG) in MS patients, in comparison to a healthy control group (14.3% vs. 34 %; 0.0129 at P≤0.05; odds ratio [OR] 3.0909, 95% confidence interval [CI]: 1.2704-7.5203). However, MS cases and controls did not differ significantly in neither GT nor TT genotypes, 62.9% (OR 0.6402, 95% CI: 0.3064-1.3374) and 52% (OR 0.5494, 95% CI: 0.2074-1.4557), respectively. The data of allele frequencies in patients and controls showed that the G allele frequencies were 0.46 vs. 0.60 in patients and controls, respectively, while T allele frequencies were 0.54 vs. 0.40 in patients and controls, respectively. The current conclusions indicated that in the study group, the GG genotype of IL-21(rs2055979) could be related to MS.

Keywords

• il-21
• rs2055979
• multiple sclerosis
• pcr-rflp