Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3

Daouda A. K. Traore; Jessica A. Wisniewski; Sarena F. Flanigan; Paul J. Conroy; Santosh Panjikar; Yee-Foong Mok; Carmen Lao; Michael D. W. Griffin; Vicki Adams; Julian I. Rood; James C. Whisstock

doi:10.1038/s41467-018-06096-2

Nature Communications (Sep 2018)

Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3

Daouda A. K. Traore,
Jessica A. Wisniewski,
Sarena F. Flanigan,
Paul J. Conroy,
Santosh Panjikar,
Yee-Foong Mok,
Carmen Lao,
Michael D. W. Griffin,
Vicki Adams,
Julian I. Rood,
James C. Whisstock

Affiliations

Daouda A. K. Traore: Department of Biochemistry and Molecular Biology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University
Jessica A. Wisniewski: Department of Microbiology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University
Sarena F. Flanigan: Department of Biochemistry and Molecular Biology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University
Paul J. Conroy: Department of Biochemistry and Molecular Biology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University
Santosh Panjikar: Department of Biochemistry and Molecular Biology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University
Yee-Foong Mok: Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne
Carmen Lao: Department of Microbiology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University
Michael D. W. Griffin: Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne
Vicki Adams: Department of Microbiology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University
Julian I. Rood: Department of Microbiology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University
James C. Whisstock: Department of Biochemistry and Molecular Biology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University

DOI: https://doi.org/10.1038/s41467-018-06096-2
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 11

Abstract

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Conjugative transfer of antibiotic resistance plasmid pCW3 in Clostridium perfringens is mediated by the tcp locus. Here, the authors identify a wHTH-type protein, TcpK, that is essential for efficient plasmid transfer and interacts with the plasmid oriT region in a unique manner.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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