Genetic Diversity and DNA Fingerprinting in Broccoli Carrying Multiple Clubroot Resistance Genes Based on SSR Markers

Abstract

To identify cultivars quickly and accurately, DNA fingerprinting of 10 broccoli varieties was performed by using simple sequence repeat (SSR) marker technology. Highly informative and polymorphic SSR markers were screened using broccoli and rapeseed. Out of the 93 SSR marker pairs, 21 pairs were selected and found to have good polymorphism. Each marker pair generated 1 to 10 polymorphic bands with an average of 4.29. The average polymorphism information content (PIC) was 0.41 with a range from 0.16 to 0.95. Six selected marker pairs established the fingerprinting of the 10 accessions and their unique fingerprints. Cluster analysis of 10 accessions showed that the genetic similarity coefficient was between 0.57 and 0.91. They can be divided into 3 groups at the genetic similarity coefficient (GSC) of 0.73. The above results indicated that DNA fingerprinting could provide a scientific basis for the identification of broccoli polymerized multiple clubroot resistance genes. Research shows that SSR marker-based DNA fingerprinting further ensures plant seed purity.

Keywords

• broccoli
• genetic diversity
• SSR markers
• fingerprinting
• clustering analysis