Feeding inhibition in daphnids - A sensitive and rapid toxicity endpoint for chemical stress?

Abstract

The planktonic Crustacea Daphnia are among the most employed organisms in ecotoxicology, mainly in regulatory assays that follow OECD/ISO protocols. The most common endpoint for acute testing (24–48 h) without feeding of organisms is usually monitored as mortality or immobilization. A rapid and physiologically and environmentally more relevant toxicity endpoint could be the impaired feeding of daphnids. Decreased feeding of test organisms upon exposure to toxicants has been used to evaluate sub-lethal effects occurring already in minutes to hours. This endpoint, however, has not been used systematically and the respective data are inconsistent due to heterogeneity of experimental design. The aim of this review is to evaluate the scientific literature where impaired Daphnia feeding has been used in ecotoxicological research. The search made in WoS (June 5, 2024) using combination of keywords “Daphni* AND feed* yielded 152 articles. Out of these 152 papers 46 addressed feeding of daphnids upon exposure to various toxicants (insecticides, heavy metals, pharmaceuticals, contaminated environmental samples and toxic cyanobacteria; in total 59 different chemicals/combinations). These 46 papers formed the basis of the critical analysis presented in the current review. For 18 chemicals it was possible to compare the sensitivity of the feeding and mortality endpoints. We conclude that although the feeding inhibition of Daphnia sp. did not prove systematically more sensitive than mortality/immobilization, it is a sub-lethal endpoint that allows rapid evaluation of toxic effects of chemicals to aquatic crustaceans – important and sensitive organisms in the aquatic food web.

Keywords

• Feeding behavior
• Daphnia sp.
• Ecotoxicity
• Sub-lethal toxicity
• Acute toxicity testing
• Microalgae