Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids

Maria C. Heinz; Koen C. Oost; Hugo J.G. Snippert

STAR Protocols (Dec 2020)

Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids

Maria C. Heinz,
Koen C. Oost,
Hugo J.G. Snippert

Affiliations

Maria C. Heinz: Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, University Utrecht, Utrecht, The Netherlands; Oncode Institute, Utrecht, The Netherlands; Correspondence
Koen C. Oost: Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, University Utrecht, Utrecht, The Netherlands; Oncode Institute, Utrecht, The Netherlands
Hugo J.G. Snippert: Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, University Utrecht, Utrecht, The Netherlands; Oncode Institute, Utrecht, The Netherlands; Correspondence

Journal volume & issue: Vol. 1, no. 3
p. 100126

Abstract

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Summary: Patient-derived organoids maintain functional and phenotypic characteristics of the original tissue such as cell-type diversity. Here, we provide protocols on how to label intestinal (cancer) stem cells by integrating the stem cell ASCL2 reporter (STAR) into human and mouse genomes via two different strategies: (1) lentiviral transduction or (2) transposon-based integration. Organoid technology, in combination with the user-friendly nature of STAR, will facilitate basic research in human and mouse adult stem cell biology.For complete details on the use and execution of this protocol, please refer to Oost et al. (2018).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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