Expression of Opa interacting protein 5 in pancreatic cancer and its effect on the proliferation of PANC-1 cells

Abstract

Objective To explore the expression of OPA interacting protein 5 (OIP5) in pancreatic cancer and its effect on the proliferation of PANC-1 cells. Methods The expression of OIP5 in pancreatic cancer and adjacent tissues was evaluated by database; the expression of OIP5 mRNA and protein in human pancreatic cancer cell lines like MIAPaCa-2, PANC-1, KP-3 and BxPC-3 was detected by real-time quantitative PCR (RT-qPCR) and Western blot, respectively; the expression of OIP5 gene silencing plasmid (pGCSIL-shOIP5)and control plasmid(pGCSIL-shCtrl)were observed, respectively infected with PANC-1 cells, then divided into OIP5 gene silencing group and shctrl control group. 5 days later, RT-qPCR and Western blot were used to detect slow virus knockdown efficiency, and flow cytometry was used to detect cell apoptosis. MTT and cell count were carried out in OIP5 gene silencing group and shctrl control group for 5 consecutive days. OIP5 gene silencing group and shctrl control group were incubated for 10 days to form colonies, then Giemsa staining was used to count total number of sets. Results The expression of OIP5 mRNA in pancreatic cancer was significantly higher than that in normal pancreatic tissue(P＜0.05), and the overall survival rate in patients with high expression of OIP5 was significantly lower than that in patients with low expression of OIP5 (P＜ 0.05), and the disease-free survival rate was also significantly lower (P＜0.05); the expression of OIP5 was higher in MIAPaCa-2, PANC-1 and KP-3, but lower in BXPC-3 cell line; the results of MTT showed that OIP5 was silenced in the 4th day. The proliferation rate of PANC-1 cells was significantly decreased on the 4th and the 5th day (P＜0.01); the number of cell colonies (9 on average) after OIP5 silencing was significantly lower than that in shctrl control group (40 on average) (P＜0.01); the apoptosis rate of PANC-1 cells after OIP5 silencing was 8.3% and 4.5% higher than that of shctrl (P＜0.01). Conclusions OIP5 is highly expressed in pancreatic cancer cell lines. OIP5 gene can regulate the proliferation, apoptosis and clonal formation of PANC-1 cells, suggesting that OIP5 may play a role as a oncogene in the pathogenesis of pancreatic cancer, thus providing a potential biomarker for targeted treatment of pancreatic cancer.

Keywords

• opa interacting protein 5|pancreatic cancer|proliferation|apoptosis