Communications Biology (Oct 2021)

ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces

  • Yamal Al-ramahi,
  • Akos Nyerges,
  • Yago Margolles,
  • Lidia Cerdán,
  • Gyorgyi Ferenc,
  • Csaba Pál,
  • Luis Ángel Fernández,
  • Víctor de Lorenzo

DOI
https://doi.org/10.1038/s42003-021-02702-0
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 11

Abstract

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Yamal Al-ramahi et al. describe an accelerated laboratory evolution method that applies ssDNA recombineering and bacterial surface display to engineer nanobody epitope recognition. As a proof-of-concept, they evolved TD4 recognition to bind the translocated intimin receptor (TirM) of EPEC pathogens, with this method bypassing the need for laborious cloning and mutagenesis procedures.