Primer synthesis by a eukaryotic-like archaeal primase is independent of its Fe-S cluster

Sandro Holzer; Jiangyu Yan; Mairi L. Kilkenny; Stephen D. Bell; Luca Pellegrini

doi:10.1038/s41467-017-01707-w

Nature Communications (Nov 2017)

Primer synthesis by a eukaryotic-like archaeal primase is independent of its Fe-S cluster

Sandro Holzer,
Jiangyu Yan,
Mairi L. Kilkenny,
Stephen D. Bell,
Luca Pellegrini

Affiliations

Sandro Holzer: Department of Biochemistry, University of Cambridge
Jiangyu Yan: Molecular and Cellular Biochemistry Department, Indiana University
Mairi L. Kilkenny: Department of Biochemistry, University of Cambridge
Stephen D. Bell: Molecular and Cellular Biochemistry Department, Indiana University
Luca Pellegrini: Department of Biochemistry, University of Cambridge

DOI: https://doi.org/10.1038/s41467-017-01707-w
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 13

Abstract

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Primase is the specialised DNA-dependent RNA polymerase responsible for the initiation of DNA synthesis during DNA replication. Here the authors use a structural biology approach to identify the initiation site in the S. solfataricus PriSLX primase and to demonstrate that its Fe-S cluster is dispensable for primer synthesis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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