Prenatal Invasive Diagnosis with Quantitative Fluorescent PCR

Abstract

Purpose: To develop the method of prenatal invasive diagnosis and compare it abilities with traditional genetic cytological examination.Materials and Methods: There was conducted prenatal fetal invasive diagnosis of 366 pregnant women. Depending on the pregnancy age and genetic indications the chorionic villus sampling, cordocentesis and amniocentesis were carried.Results: The obtained samples was treated with QF-PCR and it results was verified by standard karyotyping. In all cases, the differences of the two methods was not observed.Summary: It was shown the technique of quantitative fluorescent polymerase chain reaction (QF-PCR) has advantages over the standard cytologic examination and reduces analysis duration and increases the number of patients.

Keywords

• prenatal diagnosis
• chromosome diseases
• quantitative polymerase-chain reaction