Targeting Mice Mammary Adenocarcenoma Cells with Zinc Oxide Nanoparticles

Abstract

The cytotoxic effect of zinc oxide nanoparticles against mice mammary adenocarcenoma cells was carried out in vitro. The used nanoparticles were synthesized by pulsed laser ablation in liquid methods; the used liquid was deionized water. Colloidal suspension of zinc oxide nanoparticles was synthesized by Q-switched Nd: YAG pulse laser device (1064 nm) laser energy was 600 mJ and pulse duration was 10 nanosecond. High purity metallic zinc plate was submerged in 10 ml deionized water in 50 ml glass baker and ablated with 1000 pulse at room temperature. The nanospecificities of the synthesized nanoparticle colloidal were characterized with UV-Vis scanning spectrophotometer. Atomic force microscope was used to determine the particle size distribution and particles shape. The ZnO crestless formation was characterized by X-ray diffraction. Mice mammary adenocarcenoma cells was treated with 25, 50, 100 ppm of ZnO nanoparticles. Results indicated a significant toxicity of the ZnO nanoparticles toward cancer cells, this toxicity correlated directly with ZnO nanoparticles elevated concentrations. These result need to be further investigated and the molecular mechanism of ZnO nanoparticles effect should be more clarify.

Keywords

• nanotechnology, zinc oxide nanoparticles, anticancer, nanobiotechnology, nanomedicine