CD7 protein plays a crucial role in T cell infiltration in tumors
Binjie Sheng,
Kailu Zhang,
Shuaiyu Tian,
Renyuxue Ma,
Zixuan Li,
Hai Wu,
Tian Wang,
Licui Jiang,
Fengtao You,
Gangli An,
Huimin Meng,
Lin Yang,
Xin Liu
Affiliations
Binjie Sheng
Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China; PersonGen BioTherapeutics (Suzhou) Co., Ltd., PR China
Kailu Zhang
Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China
Shuaiyu Tian
Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China
Renyuxue Ma
Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China
Zixuan Li
Affiliated Children's Hospital of Jiangnan University (Wuxi Children's Hospital), Wuxi, Jiangsu, 214000, China
Hai Wu
Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China; PersonGen BioTherapeutics (Suzhou) Co., Ltd., PR China
Tian Wang
Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China; PersonGen BioTherapeutics (Suzhou) Co., Ltd., PR China
Licui Jiang
Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China; PersonGen BioTherapeutics (Suzhou) Co., Ltd., PR China
Fengtao You
PersonGen BioTherapeutics (Suzhou) Co., Ltd., PR China
Gangli An
Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China
Huimin Meng
PersonGen BioTherapeutics (Suzhou) Co., Ltd., PR China
Lin Yang
Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China; PersonGen BioTherapeutics (Suzhou) Co., Ltd., PR China; Corresponding author.No.199 Ren 'ai Road, Suzhou Industrial Park, Jiangsu Province, 215123, China.
Xin Liu
The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China; Corresponding author. Department of Hematology, First Affiliated Hospital of University of Science and Technology of China (Anhui Provincial Hospital), No.17 Lujiang Road, Luyang District, Hefei City, Anhui Province, 230001, China.;
CD7 protein as a target is being used to treat CD7+ lymphoma; however, the role of CD7 in the hematopoietic system remains largely unknown. Therefore, we evaluated the effects of CD7 KO in mice. The differentiation of the hematopoietic system in the bone marrow and the number of various cell types in the thymus and spleen did not differ between CD7 KO and WT mice. After subcutaneous inoculation of B16–F10 melanoma cells, tumors from CD7 KO mice grew more rapidly, and the proportion of CD8+ T cells in the spleen and tumors decreased. In vitro, the infiltration and adhesion of CD8+ T cells from the spleen of CD7 KO mice were weakened. Blocking CD7 in normal T cells did not alter the migration and infiltration, but in Jurkat, CCRF-CEM, and KG-1a tumor cell lines, migration and invasion were significantly reduced after blocking CD7. Therefore, CD7 does not affect hematopoietic system development but plays a crucial role in T cell infiltration into tumors.
