Three-dimensional wide-field fluorescence microscopy for transcranial mapping of cortical microcirculation

Quanyu Zhou; Zhenyue Chen; Yu-Hang Liu; Mohamad El Amki; Chaim Glück; Jeanne Droux; Michael Reiss; Bruno Weber; Susanne Wegener; Daniel Razansky

doi:10.1038/s41467-022-35733-0

Nature Communications (Dec 2022)

Three-dimensional wide-field fluorescence microscopy for transcranial mapping of cortical microcirculation

Quanyu Zhou,
Zhenyue Chen,
Yu-Hang Liu,
Mohamad El Amki,
Chaim Glück,
Jeanne Droux,
Michael Reiss,
Bruno Weber,
Susanne Wegener,
Daniel Razansky

Affiliations

Quanyu Zhou: Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich
Zhenyue Chen: Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich
Yu-Hang Liu: Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich
Mohamad El Amki: Department of Neurology, University Hospital and University of Zurich
Chaim Glück: Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich
Jeanne Droux: Department of Neurology, University Hospital and University of Zurich
Michael Reiss: Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich
Bruno Weber: Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich
Susanne Wegener: Department of Neurology, University Hospital and University of Zurich
Daniel Razansky: Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich

DOI: https://doi.org/10.1038/s41467-022-35733-0
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 11

Abstract

Read online

A 3D wide-field fluorescence microscopy method is introduced based on optical astigmatism combined with fluorescence source localization. It enables transcranial cortical microcirculation mapping in murine brain with high spatiotemporal resolution.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal