Ciência Animal Brasileira (Jun 2010)
COLETA DE SÊMEN OVINO EM ESTAÇÃO OU DECÚBITO LATERAL UTILIZANDO DIFERENTES ELETROEJACULADORES
Abstract
The objective of the experiment was to evaluate and comparetwo electroejaculators: an automatic one (A – Biocom Ltda.,Uberaba, MG) and a manual one (M – Duboi, Campo Grande,MS), using two methods of physical restrain: standing (E) or lateralrecumbency (D). Twelve rams were used: Santa Inês (n=9),Ile-de-France (n=2) and Texel (n=1). Semen were collected twicea week and each animal had the semen collected three times ineach group (AD – automatic in recumbency, AE – automatic instation, MD – manual in recumbency, and ME – manual in station)resulting in 12 collections. Blood samples were obtained before and after the semen collections to measure changes in circulatingcortisol in order to measure stress among groups and individuals.The collections were evaluated per the time in seconds to obtain seminalfraction, time of seminal fraction and percentage of rams thatexposed the penis. The collected semen parameters evaluated were:volume, aspect, progressive motility, wave motion, sperm vigor,total number of spermatozoa and sperm morphology. In 7.1% ofthe collections, there was no ejaculation, moreover, group AD wasresponsible for most of the collections without ejaculation (20%)(P0.05) relatingto cortisol level after collections. However, there was differenceamong individuals (P<0.05). The collections in lateral recumbencywere faster regarding achievement of seminal fraction and providedmore time length for seminal fraction. The MD group required ashorter time length to achieve the seminal fraction and produced agreater volume of ejaculate in comparison to the AS group. It canbe concluded by these results that both eletroejaculators (automaticand manual) had similar results as well as both methods of physicalrestrain (standing and lateral recumbency).
