In Vitro Assessment of Salt Stress Tolerance in Wild Potato Species
Raffaele Garramone,
Giuseppe Paolo Coppola,
Riccardo Aversano,
Teresa Docimo,
Petr Sedlák,
Domenico Carputo
Affiliations
Raffaele Garramone
Department of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Portici, Italy
Giuseppe Paolo Coppola
Department of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Portici, Italy
Riccardo Aversano
Department of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Portici, Italy
Teresa Docimo
Institute of Biosciences and Bioresources (CNR-IBBR), National Research Council of Italy, Via Università 133, 80055 Portici, Italy
Petr Sedlák
Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, 165 00 Prague, Czech Republic
Domenico Carputo
Department of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Portici, Italy
Proof of concept salt tolerance and plasticity. Wild germplasm may represent a precious source of genetic variability for salt tolerance. This study evaluated the morphological changes occurring under controlled and saline conditions in tuber-bearing S. bulbocastanum, S. commersonii, S. chomatophyllum, S. multidissectum, S. pinnactisectum, S. phureja, and cultivated S. tuberosum. An in vitro screening method was employed. Significant phenotypic variations were observed for all phenotypic traits analyzed at all NaCl levels (0, 40, 60, and 120 mM). In addition, a significant correlation between root plasticity and salt tolerance was found. Further, changes in proline and total phenolic content were assessed to envisage the metabolic adjustments of tolerant clones towards salinity. The most promising sources of tolerance were identified in S. commersonii and S. multidissectum and information obtained is discussed from a breeding perspective.
