Comparative Study of Natural Antioxidants from Glycine max, Anethum graveolensand Pimpinella anisum Seed and Sprout Extracts Obtained by Ultrasound-Assisted Extraction

Fanica Balanescu; Anna Cazanevscaia Busuioc; Andreea Veronica Dediu Botezatu; Steluta Gosav; Sorin Marius Avramescu; Bianca Furdui; Rodica Mihaela Dinica

doi:10.3390/separations9060152

Separations (Jun 2022)

Comparative Study of Natural Antioxidants from Glycine max, Anethum graveolensand Pimpinella anisum Seed and Sprout Extracts Obtained by Ultrasound-Assisted Extraction

Fanica Balanescu,
Anna Cazanevscaia Busuioc,
Andreea Veronica Dediu Botezatu,
Steluta Gosav,
Sorin Marius Avramescu,
Bianca Furdui,
Rodica Mihaela Dinica

Affiliations

Fanica Balanescu: Department of Chemistry, Physics and Environment, “Dunărea de Jos” University of Galati, 111 Domnească Street, 800201 Galati, Romania
Anna Cazanevscaia Busuioc: Department of Chemistry, Physics and Environment, “Dunărea de Jos” University of Galati, 111 Domnească Street, 800201 Galati, Romania
Andreea Veronica Dediu Botezatu: Department of Chemistry, Physics and Environment, “Dunărea de Jos” University of Galati, 111 Domnească Street, 800201 Galati, Romania
Steluta Gosav: Department of Chemistry, Physics and Environment, “Dunărea de Jos” University of Galati, 111 Domnească Street, 800201 Galati, Romania
Sorin Marius Avramescu: Faculty of Chemistry, Department of Organic Chemistry, Biochemistry and Catalysis, University of Bucharest, 90–92 Panduri Street, 050663 Bucharest, Romania
Bianca Furdui: Department of Chemistry, Physics and Environment, “Dunărea de Jos” University of Galati, 111 Domnească Street, 800201 Galati, Romania
Rodica Mihaela Dinica: Department of Chemistry, Physics and Environment, “Dunărea de Jos” University of Galati, 111 Domnească Street, 800201 Galati, Romania

DOI: https://doi.org/10.3390/separations9060152
Journal volume & issue: Vol. 9, no. 6
p. 152

Abstract

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The study aimed to evaluate the antioxidant potential of sprout and seed extracts from three species of plants, namely Glycine max (GMsp-sprouts, GMsd-seeds), Anethum graveolens (AGsp-sprouts, AGsd-seeds) and Pimpinella anisum (PAsp-sprouts, PAsd-seeds), which are widely accepted by consumers and have various applications in food flavoring, and also in natural medical treatments in the pharmaceutical industries. These plants are rich in valuable compounds that show a remarkable antioxidant power and are associated with many health benefits. Ethanol extracts were obtained by ultrasound-assisted extraction and they were comparatively evaluated for their in vitro antioxidant properties. The extracts were characterized by HPTLC, HPLC-DAD, total phenol content (TPC), total flavonoid content (TFC) analysis and antioxidant activities with different assays, such as total antioxidant capacity (TAC), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization assay (ABTS), 1,1-diphenyl 1-2-picryl-hydrazyl (DPPH) and iron binding ability of chelators. Our results showed that the sprout and seed extracts of the studied plants exhibited a high content of phytochemicals and promising antioxidant properties. The highest polyphenols content was detected for AGsd (53.02 ± 0.57 mg/g DW), PAsd (48.75 ± 0.34 mg/g DW) and the highest flavonoids content for PAsp (26.84 ± 0.57 mg/g DW). Moreover, the presence of valuable compounds was demonstrated by using HPTLC, FT-IR and HPLC-DAD techniques. In order to have a better understanding of the relationship between the biological properties and the electronic structure, a molecular modelling study of genistein was also conducted. Our approach to the comparative assessment of these three plant species was based on a priori knowledge from literature data; however, this study demonstrated that these plant extracts of seeds and also sprouts are excellent sources of natural antioxidants. Significant additional differences that were found in the phytochemical composition could be exploited in future research for pharmaceutical purposes.

Published in Separations

ISSN: 2297-8739 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Physics; Science: Chemistry
Website: http://www.mdpi.com/journal/separations

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