Sustained delivery of rhMG53 promotes diabetic wound healing and hair follicle development

Hong Niu; Haichang Li; Ya Guan; Xin Zhou; Zhongguang Li; Serana Li Zhao; Peng Chen; Tao Tan; Hua Zhu; Valerie Bergdall; Xuehong Xu; Jianjie Ma; Jianjun Guan

Bioactive Materials (Dec 2022)

Sustained delivery of rhMG53 promotes diabetic wound healing and hair follicle development

Hong Niu,
Haichang Li,
Ya Guan,
Xin Zhou,
Zhongguang Li,
Serana Li Zhao,
Peng Chen,
Tao Tan,
Hua Zhu,
Valerie Bergdall,
Xuehong Xu,
Jianjie Ma,
Jianjun Guan

Affiliations

Hong Niu: Department of Mechanical Engineering and Materials Science, Washington University in St. Louis, St. Louis, MO, 63130, USA; Institute of Materials Science and Engineering, Washington University in St. Louis, St. Louis, MO, 63130, USA
Haichang Li: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, 43210, USA
Ya Guan: Department of Mechanical Engineering and Materials Science, Washington University in St. Louis, St. Louis, MO, 63130, USA; Institute of Materials Science and Engineering, Washington University in St. Louis, St. Louis, MO, 63130, USA
Xin Zhou: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, 43210, USA; Laboratory of Cell Genetics and Developmental Biology, Shaanxi Normal University College of Life Sciences, Xi'an, 710062, China
Zhongguang Li: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, 43210, USA
Serana Li Zhao: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, 43210, USA
Peng Chen: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, 43210, USA
Tao Tan: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, 43210, USA
Hua Zhu: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, 43210, USA
Valerie Bergdall: Department of Veterinary Preventive Medicine, University Laboratory Animals Resources, The Ohio State University, Columbus, OH, 43210, USA
Xuehong Xu: Laboratory of Cell Genetics and Developmental Biology, Shaanxi Normal University College of Life Sciences, Xi'an, 710062, China
Jianjie Ma: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, 43210, USA; Corresponding author.
Jianjun Guan: Department of Mechanical Engineering and Materials Science, Washington University in St. Louis, St. Louis, MO, 63130, USA; Institute of Materials Science and Engineering, Washington University in St. Louis, St. Louis, MO, 63130, USA; Corresponding author. Department of Mechanical Engineering and Materials Science, Washington University in St. Louis, St. Louis, MO, 63130, USA.

Journal volume & issue: Vol. 18
pp. 104 – 115

Abstract

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MG53 is an essential component of the cell membrane repair machinery, participating in the healing of dermal wounds. Here we develop a novel delivery system using recombinant human MG53 (rhMG53) protein and a reactive oxygen species (ROS)-scavenging gel to treat diabetic wounds. Mice with ablation of MG53 display defective hair follicle structure, and topical application of rhMG53 can promote hair growth in the mg53−/− mice. Cell lineage tracing studies reveal a physiological function of MG53 in modulating the proliferation of hair follicle stem cells (HFSCs). We find that rhMG53 protects HFSCs from oxidative stress-induced apoptosis and stimulates differentiation of HSFCs into keratinocytes. The cytoprotective function of MG53 is mediated by STATs and MAPK signaling in HFSCs. The thermosensitive ROS-scavenging gel encapsulated with rhMG53 allows for sustained release of rhMG53 and promotes healing of chronic cutaneous wounds and hair follicle development in the db/db mice. These findings support the potential therapeutic value of using rhMG53 in combination with ROS-scavenging gel to treat diabetic wounds.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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