Nature Communications (May 2022)
Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection
Abstract
Faster cryo specimen preparation can advance cryo electron microscopy (cryoEM). Here, the authors present a vitrification device with automated sample handling for cryoEM of proteins, suspensions and cells, enabling blot-free sample thinning, dew-point control and characterization of cryo grids prior to data acquisition.