Tracking Electrons in Biological Macromolecules: From Ensemble to Single Molecule

Abstract

Nature utilizes oxido-reductases to cater to the energy demands of most biochemical processes in respiratory species. Oxido-reductases are capable of meeting this challenge by utilizing redox active sites, often containing transition metal ions, which facilitate movement and relocation of electrons/protons to create a potential gradient that is used to energize redox reactions. There has been a consistent struggle by researchers to estimate the electron transfer rate constants in physiologically relevant processes. This review provides a brief background on the measurements of electron transfer rates in biological molecules, in particular Cu-containing enzymes, and highlights the recent advances in monitoring these electron transfer events at the single molecule level or better to say, at the individual event level.

Keywords

• electron transfer
• random sequential
• single molecule
• enzyme
• FRET
• redox
• nitrite reductase
• small laccase
• copper protein
• ABEL trap