EXPERIMENTAL RESULTS OF RAT HYALINE CARTILAGE SURFACE DEFECT REPLACEMENT WITH A CELL ENGINEERING STRUCTURE

Abstract

Nowadays, hyaline cartilage damage therapy and repair is one of the most topical research fields in regenerative medicine. however, in spite of a large number of methods used in clinical practice, this problem has not been fully solved yet. a promising approach in dealing with this problem may be a transplantation of a cell engineering structure (CES), made up of cell culture and biodegradable scaffold (membrane). In our experiments with animal models we used a membrane made of polylactide and multipotent mesenchymalstem cells (MSC) culture, and the damage of the knee joint hyaline layer was simulated by dental boron. The cell culture was pre-modified by TGF-β-3 growth factor to promote the synthesis of extracellular matrix proteins (ECM). as a result, we observed a fourfold increase in Acan and col2a1 expression compared to the control. Damaged areas were analyzed by scanning electron microscopy (SEM) over long observation periods (up to 90 days), and the diameter of the damaged area was found to decrease after CES transplantation, while the damage area in the control group, without CES, increased more than 2.5-fold in diameter. Thus, application of cell engineering structures is a promisingmethod of repairing the damaged layer of hyaline cartilage. however, further studies are needed to enhance the effectiveness of the cell culture modification, develop methods for combining it with biodegradable scaffold, and select the method of fixing the CES in the damaged area.

Keywords

• гиалиновый хрящ
• дефект
• клеточно-инженерная конструкция
• восстановление
• ммск