This study aimed to investigate how diets supplemented with DHA-rich algae affect the expression of liver lipid synthesis genes in dairy goat bucks. The results revealed that when supplemented with DHA-rich algae, liver weight and serum HDL-C were significantly increased (p p SCD, FADS1, INSIG1), de novo synthesis fatty acids (FASN), fatty acid transport (LDLR), and cholesterol and steroid synthesis (HMGCR, HMGCS1, SQLE) genes were significantly increased (p ALDH3B1) genes were significantly decreased (p < 0.05). In conclusion, this research provided preliminary evidence that supplementation with algae in dietary supplements altered the expression of the liver lipid synthesis genes in the Saanen dairy goat bucks.