Pathway of Diatoms Enter Experimental Rabbits through the Lymphatic System of the Digestive Tract

Abstract

ObjectiveTo study whether diatoms can enter the body through the lymphatic system of the digestive tract.MethodsTwenty experimental rabbits were divided into the test group and the control group randomly, and intragastric administration was performed with 20 mL water sample from the Pearl River and 20 mL ultrapure water, respectively. After 30 min, lymph, lungs， livers and kidneys were extracted for the diatom test. The concentration, size and type of diatoms were recorded.ResultsThe concentration of diatoms of the test group was higher than that of the control group （P<0.05）. In the test group, Stephanodiscus, Coscinodiscus, Cyclotella, Melosira， Nitzschia， Synedra， Cymbella, and Navicula were detected; in the control group， Stephanodiscus, Coscinodiscus and Cyclotella were detected. The long diameter and the short diameter of diatoms of the test group were higher than those of the control group （P<0.05）. In the test group, 1-2 diatoms were detected in 3 lung samples and 2 liver samples, which were Stephanodiscus or Cyclotella, and no diatoms were detected in the kidney samples; in the control group, 1-2 diatoms were detected in 2 lung samples and 3 liver samples, which were Stephanodiscus or Coscinodiscus, and no diatoms were detected in the kidney samples.ConclusionDiatoms can enter the body through the lymphatic fluid, which is one of the reasons for the presence of diatoms in tissues and organs of non-drowning cadavers.

Keywords

• forensic pathology
• drowning
• diatom
• digestive tract
• lymph
• gavage model