Nature Communications (Jun 2017)
Phosphate steering by Flap Endonuclease 1 promotes 5′-flap specificity and incision to prevent genome instability
- Susan E. Tsutakawa,
- Mark J. Thompson,
- Andrew S. Arvai,
- Alexander J. Neil,
- Steven J. Shaw,
- Sana I. Algasaier,
- Jane C. Kim,
- L. David Finger,
- Emma Jardine,
- Victoria J.B. Gotham,
- Altaf H. Sarker,
- Mai Z. Her,
- Fahad Rashid,
- Samir M. Hamdan,
- Sergei M. Mirkin,
- Jane A. Grasby,
- John A. Tainer
Affiliations
- Susan E. Tsutakawa
- Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory
- Mark J. Thompson
- Department of Chemistry, Centre for Chemical Biology, Sheffield Institute for Nucleic Acids (SInFoNiA), University of Sheffield
- Andrew S. Arvai
- Department of Molecular Biology, The Scripps Research Institute
- Alexander J. Neil
- Department of Biology, Tufts University
- Steven J. Shaw
- Department of Chemistry, Centre for Chemical Biology, Sheffield Institute for Nucleic Acids (SInFoNiA), University of Sheffield
- Sana I. Algasaier
- Department of Chemistry, Centre for Chemical Biology, Sheffield Institute for Nucleic Acids (SInFoNiA), University of Sheffield
- Jane C. Kim
- Department of Biology, Tufts University
- L. David Finger
- Department of Chemistry, Centre for Chemical Biology, Sheffield Institute for Nucleic Acids (SInFoNiA), University of Sheffield
- Emma Jardine
- Department of Chemistry, Centre for Chemical Biology, Sheffield Institute for Nucleic Acids (SInFoNiA), University of Sheffield
- Victoria J.B. Gotham
- Department of Chemistry, Centre for Chemical Biology, Sheffield Institute for Nucleic Acids (SInFoNiA), University of Sheffield
- Altaf H. Sarker
- Biological Systems and Engineering, Lawrence Berkeley National Laboratory
- Mai Z. Her
- Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory
- Fahad Rashid
- Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology
- Samir M. Hamdan
- Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology
- Sergei M. Mirkin
- Department of Biology, Tufts University
- Jane A. Grasby
- Department of Chemistry, Centre for Chemical Biology, Sheffield Institute for Nucleic Acids (SInFoNiA), University of Sheffield
- John A. Tainer
- Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory
- DOI
- https://doi.org/10.1038/ncomms15855
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 15
Abstract
Flap Endonuclease 1 is a DNA replication and repair enzyme indispensable for maintaining genomic stability. Here the authors provide mechanistic details on how FEN1 selects for 5′-flaps and promotes catalysis to avoid large-scale repeat expansion by a process termed ‘phosphate steering’.
