A case of chronic wounds caused by Sporothrix schenckii infection was rapidly detected by metagenomic next generation sequencing
Wenxiang Jin,
Yong Liu,
Qiuyue Ning,
Shuwen Wu,
Sibiao Su,
Dongyan Zheng,
Shasha Ma,
Jun Zou,
Min Yang,
Diefei Hu,
Huarong Ding
Affiliations
Wenxiang Jin
Department of Infectious Diseases, KingMed Diagnostics, Guangzhou, Guangdong, 510005, China
Yong Liu
Department of Infectious Diseases, KingMed Diagnostics, Guangzhou, Guangdong, 510005, China
Qiuyue Ning
Department of Infectious Diseases, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, China
Shuwen Wu
Department of Infectious Diseases, KingMed Diagnostics, Guangzhou, Guangdong, 510005, China
Sibiao Su
Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, China
Dongyan Zheng
Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, China
Shasha Ma
Department of Infectious Diseases, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, China
Jun Zou
Department of Infectious Diseases, The Fourth People's Hospital of Nanning, Nanning, Guangxi, 530023, China
Min Yang
Department of Infectious Diseases, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, China; Corresponding author.
Diefei Hu
Department of Infectious Diseases, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, China; Corresponding author.
Huarong Ding
Department of Burns and Plastic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, China; Corresponding author.
The dimorphic fungus Sporothrix schenckii is widely distributed in soil, vegetation, and decaying organic matter, and can cause sporotrichosis when the patient's skin trauma was exposed to contaminated material with Sporothrix spp. The cases of Sporothrix schenckii infection in chronic wounds are rarely reported. Here we reported a 53-year-old male construction worker who was admitted to our hospital on July 9, 2022, without underlying disease presented with a painless subcutaneous hard nodule on his right calf, which later ulcerated and oozed, with an enlarged wound and no fever during the course of the disease. His procalcitonin, C-reactive protein, erythrocyte sedimentation rate increased, and necrotic histopathology suggested chronic granulomatous inflammation. Then his necrotic tissue and pus were sent for metagenomic next generation sequencing(mNGS), the result reported Sporothrix schenckii after 43 hours, which was consistent with the result of culture after 18 days. mNGS might be more useful and valuable in diseases such as sporotrichosis where it is difficult to see the yeast cells in the tissues.
