Low-Temperature Gas Plasma Combined with Antibiotics for the Reduction of Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilm Both In Vitro and In Vivo

Li Guo; Lu Yang; Yu Qi; Gulimire Niyazi; Jianbao Zheng; Ruobing Xu; Xusong Chen; Jingye Zhang; Wang Xi; Dingxin Liu; Xiaohua Wang; Hailan Chen; Michael G. Kong

doi:10.3390/life11080828

Life (Aug 2021)

Low-Temperature Gas Plasma Combined with Antibiotics for the Reduction of Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilm Both In Vitro and In Vivo

Li Guo,
Lu Yang,
Yu Qi,
Gulimire Niyazi,
Jianbao Zheng,
Ruobing Xu,
Xusong Chen,
Jingye Zhang,
Wang Xi,
Dingxin Liu,
Xiaohua Wang,
Hailan Chen,
Michael G. Kong

Affiliations

Li Guo: Center for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, China
Lu Yang: School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
Yu Qi: Center for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, China
Gulimire Niyazi: School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
Jianbao Zheng: Department of General Surgery, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
Ruobing Xu: School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
Xusong Chen: Center for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, China
Jingye Zhang: Center for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, China
Wang Xi: Center for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, China
Dingxin Liu: Center for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, China
Xiaohua Wang: Center for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, China
Hailan Chen: Frank Reidy Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA
Michael G. Kong: Center for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, China

DOI: https://doi.org/10.3390/life11080828
Journal volume & issue: Vol. 11, no. 8
p. 828

Abstract

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Biofilm infections in wounds seriously delay the healing process, and methicillin-resistant Staphylococcus aureus is a major cause of wound infections. In addition to inactivating micro-organisms, low-temperature gas plasma can restore the sensitivity of pathogenic microbes to antibiotics. However, the combined treatment has not been applied to infectious diseases. In this study, low-temperature gas plasma treatment promoted the effects of different antibiotics on the reduction of S. aureus biofilms in vitro. Low-temperature gas plasma combined with rifampicin also effectively reduced the S. aureus cells in biofilms in the murine wound infection model. The blood and histochemical analysis demonstrated the biosafety of the combined treatment. Our findings demonstrated that low-temperature gas plasma combined with antibiotics is a promising therapeutic strategy for wound infections.

Published in Life

ISSN: 2075-1729 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science
Website: http://www.mdpi.com/journal/life

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