Genetic study of Gardnerella vaginalis virulence factors isolated from vaginal discharge

Abstract

Background: Vaginitis is the most prevalent infectious condition affecting the female genital system. Prevalent vaginal infections, namely, bacterial vaginosis. Gardnerella vaginallis is one abacterial genus almost always associated with bacterial vaginosis. Objective: Isolation and identification of G. vaginalis. and Diagnosis some virulence such as Vaginolysin, Sialidase, Phospholipase. Materials and Methods: Collected 100 vaginal samples from women then cultures in Columbia blood agar to isolated G. vaginalis and genetic detection some virulence factors. Results: The findings revealed that 12 (12%) of the samples tested positive for G. vaginalis., including (7%, 26%, 13%, 8%, and 2% isolated from 20 to 50 years age, respectively. The results of PCR showed that out of 12 isolates only 4 (33%), 9 (75%) and, 6 (50%) of G. vaginalis isolates gave positive results to vly, sld and, pho gene respectively gave positive results from 12 G. vaginalis isolates. Conclusions: A healthy vaginal microbiota is considered to be significant for maintaining vaginal health and preventing infections. Bacterial vaginosis is the condition that occurs when the balance of the vaginal flora is dysregulated, and replaced by pathogenic bacteria in the vagina like G. vaginalis.

Keywords

• ardnerellaa vaginalis
• columbia blood agar
• vaginal infections