Avicenna Journal of Phytomedicine (Feb 2017)

In vitro and in vivo assessment of inhibitory effect of stevioside on pro-inflammatory cytokines

  • Jatuporn Noosud,
  • Narissara Lailerd,
  • Autchara Kayan,
  • Chaiwat Boonkaewwan

DOI
https://doi.org/10.22038/ajp.2016.6599
Journal volume & issue
Vol. 7, no. 2
pp. 101 – 106

Abstract

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Objective: Stevioside is a natural non-caloric sweetener which has been reported to have anti-inflammatory activity. The aim of the present study was to examine in vitro and in vivo effects of stevioside on rats plasma levels of tumor necrosis factor- α (TNF-α), interleukin-1β (IL-1β), TNF-α and IL-1β release from lipopolysaccharide(LPS)-stimulated rat peripheral blood mononuclear cells (PBMCs). Materials and Methods: Male wistar rats weighing between 170-220 g were given stevioside (0, 500 and 1000 mg/kg BW/day) for 6 weeks. Mononuclear cells were separated from peripheral blood samples. TNF-α and IL-1β levels in plasma and the release of TNF-α and IL-1β from PBMCs were determined using rat enzyme-linked immunosorbent assay (ELISA) kits. Results: Plasma levels of TNF-α and IL-1β were found to be non-detectable in control and groups treated with 500 and 1000 mg/kg of stevioside. Regarding TNF-α release from LPS-stimulated PBMCs, rats that were orally fed with 500 and 1000 mg/kg of stevioside were significantly different (p

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