Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium

Florian Bonkhofer; Rossella Rispoli; Philip Pinheiro; Monika Krecsmarik; Janina Schneider-Swales; Ingrid Ho Ching Tsang; Marella de Bruijn; Rui Monteiro; Tessa Peterkin; Roger Patient

doi:10.1038/s41467-019-11423-2

Nature Communications (Aug 2019)

Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium

Florian Bonkhofer,
Rossella Rispoli,
Philip Pinheiro,
Monika Krecsmarik,
Janina Schneider-Swales,
Ingrid Ho Ching Tsang,
Marella de Bruijn,
Rui Monteiro,
Tessa Peterkin,
Roger Patient

Affiliations

Florian Bonkhofer: Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Rossella Rispoli: Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Philip Pinheiro: Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Monika Krecsmarik: Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Janina Schneider-Swales: Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Ingrid Ho Ching Tsang: Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Marella de Bruijn: Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Rui Monteiro: Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Tessa Peterkin: Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Roger Patient: Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford

DOI: https://doi.org/10.1038/s41467-019-11423-2
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

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HSCs emerge from haemogenic endothelium (HE) in the dorsal aorta but whether these tissues share a common lineage is unclear. Here, the authors use a zebrafish runx1 reporter to show that HE maintains an arterial gene expression profile in the absence of Runx1, suggesting the aortic endothelium as a precursor of HE.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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