Orthopedic Research and Reviews (Jun 2022)
The Effect of Platelet-Rich Plasma on Type I Collagen Production, VEGF Expression, and Neovascularization after Femoral Bone Implants: A Study on Rat Models
Abstract
Safrizal Rahman,1,2 Endang Sutedja,3 Onarisa Ayu,1,2 Mirnasari Amirsyah4,5 1Division of Orthopedics and Traumatology, Department of Surgery, School of Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia; 2Division of Orthopedics and Traumatology, Department of Surgery, School of Medicine, Dr. Zainoel Abidin Hospital, Banda Aceh, Indonesia; 3Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; 4Division of Plastic and Reconstruction Surgery, Department of Surgery, School of Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia; 5Division of Plastic and Reconstruction Surgery, Department of Surgery, School of Medicine, Dr Zainoel Abidin Hospital, Banda Aceh, IndonesiaCorrespondence: Safrizal Rahman, Division of Orthopedics and Traumatology, Department of Surgery, School of Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia, Email [email protected]: Platelet-rich plasma (PRP) contains many growth factors, such as FGF, which induces the production of type I collagen, and VEGF, which induces neovascularization, all of which are important in bone healing. This study aimed to evaluate the effect of PRP administration on type I collagen production, VEGF expression, and neovascularization in rat models following femoral bone implants using K-wire.Methods: An experimental randomized control study was conducted on 24 white male rats (Rattus norvegicus) in the Wistar strain that underwent K-wire implantation, where PRP was administered to the treatment groups. The amount of type I collagen was measured by immunohistochemistry VEGF expression using sandwich ELISA, and neovascularization by histopathological examination.Results: The amount of type I collagen in the treatment group (50–> 150/field of view) was significantly higher than the control group (0– 99/field of view; p=0.003). VEGF expression in the treatment groups was significantly higher than controls: 10.90± 4.47 and 2.29± 0.92, respectively (p=0.006). Mean number of new vessels formed on fibrotic capsules in the treatment groups was significantly (p=0.007) higher than the control groups (2.69± 1.03 vs 0.67± 0.52).Conclusion: The use of PRP significantly increased type I collagen production, VEGF expression, and neovascularization in rat models, elucidating the potential of PRP to be used in clinical settings to enhance the bone-healing process.Keywords: platelet-rich plasma, vascular endothelial growth factor, type I collagen, neovascularization, bone healing, orthopedic implants
