Effect of Double Endotamponade with Perfluorocarbon Liquid and Silicone Oil: Experimental Study in vivo

Abstract

Purpose. To examine how the retina is affected by double tamponade of the vitreous cavity with the combination of perfluorocarbon liquid (PFCL) and silicone oil (SO) in order to assess the prospect of such double tamponade employment in clinical practice, particularly in cases of retinal detachment.Material and methods. We examined retinal response on combination of perfluorodecalin (PFD) «Dk-line» (Bausch & Lomb) and silicone oil «Oxane 1300» (Bausch & Lomb) during long term vitreous cavity tamponade. The study included 90 chinchilla rabbits (90 eyes). Rabbits were randomized into three groups: I (test group) - vitreous cavities of right eyes were filled with PFD+SO; II (control) - the same but with SO only; III (control) - the same but with PFD only. Rabbits of each group were euthanized after 1, 2, or 3 months. We performed clinical examination and morphological examination of the rabbit retinas.Results. At 1 month, retina in all groups did not differ from the normal morphology. At 2 months in all groups we detected retinal edema. At 3 months in all groups retina has thinned. According to clinical findings and morphological study we found no difference between eyes filled with PFD+SO combination and eyes filled with SO.Conclusion. No signs of pathological response were found in the retinal morphology after 1 month of PFD+SO combination stay in vitreous cavity, thus representing the absence of any risk of double tamponade during 1 month. During a longer-term tamponade of the vitreous cavity this combination is safe to the same extent as SO is.

Keywords

• double tamponade
• vitreous cavity
• complicated retinal detachments
• perfluorocarbon liquids
• perfluorodecalin
• silicone oil