Molecular Phylogeny of Edge Hill Virus Supports its Position in the Yellow Fever Virus Group and Identifies a New Genetic Variant

Joanne Macdonald; Michael Poidinger; John S. Mackenzie; Richard C. Russell; Stephen Doggett; Annette K. Broom; Debra Phillips; Joseph Potamski; Geoff Gard; Peter Whelan; Richard Weir; Paul R. Young; Debra Gendle; Sheryl Maher; Ross T. Barnard; Roy A. Hall

doi:10.4137/EBO.S4966

Evolutionary Bioinformatics (Jan 2010)

Molecular Phylogeny of Edge Hill Virus Supports its Position in the Yellow Fever Virus Group and Identifies a New Genetic Variant

Joanne Macdonald,
Michael Poidinger,
John S. Mackenzie,
Richard C. Russell,
Stephen Doggett,
Annette K. Broom,
Debra Phillips,
Joseph Potamski,
Geoff Gard,
Peter Whelan,
Richard Weir,
Paul R. Young,
Debra Gendle,
Sheryl Maher,
Ross T. Barnard,
Roy A. Hall

Affiliations

Joanne Macdonald: Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Qld. 4072, Australia.
Michael Poidinger: Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Qld. 4072, Australia.
John S. Mackenzie: Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Qld. 4072, Australia.
Richard C. Russell: Department of Medical Entomology, The University of Sydney, Westmead Hospital, Westmead, NSW, 2145, Australia.
Stephen Doggett: Department of Medical Entomology, The University of Sydney, Westmead Hospital, Westmead, NSW, 2145, Australia.
Annette K. Broom: Discipline of Microbiology and Immunology, School of Biomedical, Biomolecular, and Chemical Sciences, The University of Western Australia, Nedlands, Western Australia 6009, Australia.
Debra Phillips: Public Health Virology, Queensland Health Forensic and Scientific Services, Coopers Plains, Qld. 22222, Australia.
Joseph Potamski: Public Health Virology, Queensland Health Forensic and Scientific Services, Coopers Plains, Qld. 22222, Australia.
Geoff Gard: A.L. Rose Laboratory, Berrimah Veterinary Laboratories, Berrimah Farm, Berrimah, NT. 0828, Australia.
Peter Whelan: Medical Entomology, Centre for Disease Control, NT Department of Health and Families, Darwin, NT. 0811, Australia.
Richard Weir: A.L. Rose Laboratory, Berrimah Veterinary Laboratories, Berrimah Farm, Berrimah, NT. 0828, Australia.
Paul R. Young: Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Qld. 4072, Australia.
Debra Gendle: Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Qld. 4072, Australia.
Sheryl Maher: Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Qld. 4072, Australia.
Ross T. Barnard: Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Qld. 4072, Australia.
Roy A. Hall: Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Qld. 4072, Australia.

DOI: https://doi.org/10.4137/EBO.S4966
Journal volume & issue: Vol. 6

Abstract

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Edge Hill virus (EHV) is a mosquito-borne flavivirus isolated throughout Australia during mosquito surveillance programs. While not posing an immediate threat to the human population, EHV is a taxonomically interesting flavivirus since it remains the only member of the yellow fever virus (YFV) sub-group to be detected within Australia. Here we present both an antigenic and genetic investigation of collected isolates, and confirm taxonomic classification of the virus within the YFV-group. Isolates were not clustered based on geographical origin or time of isolation, suggesting that minimal genetic evolution of EHV has occurred over geographic distance or time within the EHV cluster. However, two isolates showed significant differences in antigenic reactivity patterns, and had a much larger divergence from the EHV prototype (19% nucleotide and 6% amino acid divergence), indicating a distinct subtype or variant within the EHV subgroup.

Published in Evolutionary Bioinformatics

ISSN: 1176-9343 (Online)
Publisher: SAGE Publishing
Country of publisher: United States
LCC subjects: Science: Biology (General): Evolution
Website: https://journals.sagepub.com/home/evb

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