Effect of Dimethylacetamide Concentration on Motility, Quality, Antioxidant Biomarkers, Anti-Freeze Gene Expression, and Fertilizing Ability of Frozen/Thawed Rooster Sperm
Gamal M. K. Mehaisen,
Ahmed M. Elomda,
Shaimaa K. Hamad,
Mona M. Ghaly,
Yanyan Sun,
Yunlei Li,
Yunhe Zong,
Jilan Chen,
Agnieszka Partyka,
Ali Nazmi,
Ahmed O. Abbas,
Farid K. R. Stino
Affiliations
Gamal M. K. Mehaisen
Department of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
Ahmed M. Elomda
Department of Animal Biotechnology, Animal Production Research Institute, Agriculture Research Center, Dokki, Giza 12572, Egypt
Shaimaa K. Hamad
Department of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
Mona M. Ghaly
Department of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
Yanyan Sun
Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Yunlei Li
Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Yunhe Zong
Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Jilan Chen
Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Agnieszka Partyka
Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland
Ali Nazmi
Department of Animal Sciences, The Ohio State University, Columbus, OH 43013, USA
Ahmed O. Abbas
Department of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
Farid K. R. Stino
Department of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
Sperm cryopreservation is of great importance for the poultry industry but still needs to be optimized. The high susceptibility of poultry sperm to cryodamage leads to low fertility rates after cryopreservation. Therefore, the present study aimed at evaluating the effect of including a cryoprotectant, dimethylacetamide (DMA), in the chicken semen freezing extenders at a final concentration of 3%, 6%, or 9% on the post-thawed sperm motility, quality, antioxidant biomarkers, anti-freeze gene expression, and fertilizing ability. Results showed that the total motile sperm, progressivity, and viability were quadratically increased (p p HSP70) and ras homolog family member A (RHOA) were linearly and quadratically down-regulated (p < 0.05) with the high concentration of DMA. Finally, the fertility and hatchability rates did not indicate statistical differences between DMA groups. It can be concluded that using the low concentration of 3–6% DMA in the freezing semen extender is preferable to obtain acceptable results in the post-thawed sperm quality and fertility.
